Proliferation-inhibiting And Differentiation-inducing Effects Of Cucurmosin On B16 Melanoma Cells

Objective: This study was designed to investigate the proliferation-inhibiting and differentiation-inducing effects of cucurmosin on murine B16 melanoma cells, in order to confirm the anticancer effect of cucurmosin and clarify its possible mechanism. To provide some theoretical foundation on anticancer mechanism of cucurmosin and its analogues, and provide a guide to differentiation-inducing therapy for cancer.Methods:â‘´Microculture tetrazolium(MTT) assay was used to evaluate the inhibitory proliferation effect in murine B16 melanoma cells after treated with different concentration of cucurmosin(1.25mg·L^-1, 2.5mg·L^-1, 5.0mg·L^-1, 10.0mg·L^-1, 20.0mg·L^-1 and 40.0mg·L^-1, respectively) for 24h, 48h and 72h.⑵B16 melanoma cells were inoculated into the C57BL/6J mice to establish B16 melanoma model, in order to investigate the effect of cucurmosin on tumor growth in C₅₇BL/6J mice with transplanting tumor of melanoma. Fifty C57BL/6J mice with melanoma tumor were randomized five groups: negative control group, cucurmosin high dose group, cucurmosin middle dose group, cucurmosin low dose group and positive CTX group. After 12 days'treatment, samples of tumor were collected and weighed.⑶To estimate differentiation-inducing activity of cucurmosin on B16 melanoma cells, morphological changes were observed and analyzed by light microscopy and electronic microscopy; distribution of cell cycle were analyzed by flow cytometry; melanin content were measured by enzyme...