Proteomic Analysis Of Differential Protein Expression During Neuronal Apoptosis Reveals MAP1B Is Substrate Of Caspase And Calpain

ObjectiveTo analyse differential protein expression during neuronal apoptosis at proteomic level, to analyse to search the substrates of caspase or calpain, and to originally find out novel proteins regulating neuronal apoptosis.Methods1. Proteomic analysis of differential protein expression during neuronal apoptosis1.1 Estabilishment of apoptotic model of cerebellar granule neurons Cerebellar granule neurons, with the 25mmol/L potassium chloride (25K) treated group as a control and the 5mmol/L potassium chloride (5K) treated group as the apoptosis inducing group, were kept in culture for 7 days.1.2 Two-dimensional difference in gel electrophoresis (2D-DIGE) analysis The total protein was extracted, purified and quantified from both groups, then...