The Expression Of Hepatocellular/Placenta FXR And BSEP In Pregnant Rats With Different Dose Estradiol Benzoate Induced

Objective: To study the expression level of FXR and BSEP in hepatocellular/placental tissue in late pregnancy rats with different dose estradiol benzoate(EB)induced.Methods: 75 SD rats pregnant for 15 days were randomly divided into five groups: control group, group A, group B, group C and group D,and given the subcutaneously injection of estradiol benzoate(EB)or appropriate volume of NS for 5 days.In control group, the rats were given NS,In group A,B,C,D, the rats received EB at a dose of 1mg/kg.d,2.5mg/kg.d,3.5mg/kg.d,5mg/kg.d respectively; venous blood was sampled for the measurement of serum ALT,AST,ALP,TBA level for twice(before given medication,before delivery). Liver and placenta were taken for histological analysis with light microscope by H&E staining after delivery and the physiological index of the fetal rats has entering into statistic. The expression of FXR/BSEP in liver and placenta was analyzed by immunohistochemical method (SP).