Construction Of The Recombinant Adenovirus Encoding Hyper-IL-6, And The Research About Ad-HIL-6 On Promoting Proliferation Of Hepatocyte

Objected : To construct the fusion gene Hyper-IL-6,and the adenoviral vector that expresses Hyper-IL-6. Detect the expression of Hyper-IL-6 in the human hepatic cell lines L02 treated with Ad-HIL-6。Mothods:Using GeneSOEing method,a cassette consisting of human sIL-6R cDNA and IL-6 cDNA was fused by a linker coding for sequences rich in glycine by PCR technique and coloned into pEGFp-c1。And The Hyper-IL-6 gene was coloned to the shuttle plasmid pAdTrack-CMV。The linearized shuttle plasmids were co-transformed with adenoviral backbone vector to E.coli BJ5183 cells。Before transfection, the recombinant adenoviral plasmids were digested with PacI。Then the digested recombinant adenoviral plasmids were transfected to 293 cells with...