| PART ONE THE PROTECTIVE EFFECT OF ULINASTATIN ON LPS-INDUCED ACUTE LUNG INJURY IN MICEObjective To investigate the protective effect of ulinastatin on LPS-induced acute lung injury and its possible mechanism. Methods Fifty mice were randomly divided into 2 groups: LPS (20mg/kg) group; LPS+UTI (1×105u/kg) group. The ALI model was set up by intraperineal injection of lipopolysaccharide (LPS). When injected LPS, the chests of mice in two groups were open at 0h,0.5h,2h,4h and 8h. Blood samples were taken through heart puncture for blood gas analysis. The changes of TNF-α,IL-8,IL-10 levels in the pulmonary tissues were detected by ELISA at defferent times.Besides, the following changes were observed: lung wet/dry weight ratio, lung histological manifestations.Results The levels of inflammatory factors in two groups mice all took on a ascending trend. TNF-α,IL-8 and W/D levels were lower significantly in UTI group than those in LPS group(P<0.05). However,...
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