Study On Proteomics Of Secreted Proteins Of Mouse Hepatoma Cell Line H₂₂

Objective The technique and strategy of proteomics was used to differentiate and identify proteins secreted by mouse hepatoma cell line H22 and normal mouse hepatocyte, and search different proteins between them.Methods Mouse hepatoma cell line H22 and primary normal mature mouse hepatocyte were cultured in serum-free medium. We collected the supernatant of serum-free medium of normal mouse hepatocyte and mouse hepatoma cell line H22, freezed drying them, then filtered them with chromatographic column and precipitated them with acetone for desalting, 2-DE and one-dimensional SDS-PAGE electrophorisis were performed to separate the proteins of treated samples, stained gel with AgNO3, PDQuestsoftware analysised image of gel, searched differentially expressed. protein spots.Results 1. Acquire primary cultured normal mouse hepatocyte in vitro, under light microscope conjugate nuclei is clear, under electron microscope mitochondrium is abundant, its cristae assume lamellar or shape of wheel, and quantity of endoplasmic reticulum is plentiful . 2.