Hydrogen Peroide Induced Oxidative Apoptosis Of Alveolar Type Ⅱ Epithelial Cell And The Regulation Mechanism Mediated By Extracellular Signal-Regulated Kinase (ERK)

Objective Oxygen therapy is widely used to improve blood oxygen saturation and reduce tissue hypoxia for patients.However,supplemental O₂ of prolonged high concentration administered to patients may result in oxidative lung injury. Alveolar type ATâ…¡epithelial cell (ATâ…¡cell) can function as a progenitor for regeneration after lung injury. Extracellular signal-regulated kinase (ERK) plays a role in cell proliferation, survival and apoptosis, which may have effect on survival or death of ATâ…¡cells under oxidative stress. In the present study, we investigated the survival and apoptotic responses of primary cultured rat ATâ…¡cells induced by hydrogen peroxide (H₂O₂) and the regulation mechanism mediated by ERK.Methods Rat ATâ…¡cells were isolated by trypsin digestion and differential adherence to plates coated with IgG. Primary cultured rat ATâ…¡cells were then stimulated by H₂O₂ to simulate ROS attack in vivo. Cells were divided into control group(cells in serum-free culture medium) ,...