| Objective: To investigate the differentiation-inducing effects of all-trans-retinoic acid (ATRA) to HPV16 subgenes-immortalized human endocervical cells (H8 cells) in vitro.Methods: HPV16 subgenes-immortalized human endocervical cells (H8 cells) were cultured in vitro. After treated with ATRA, the proliferation of immortalized human endocervical cells was measured by MTT assay; morphological changes were observed using LM and TEM; cell cycle was analyzed by FCM; expression of Ki67 was tested by immunocytochemistry and the activity of telomerase was tested using PCR-ELISA; expression of HPV and E6 antigen was tested by fluorescence immunocytochemistry.Results: (1) 10-5mol/L, 10-6mol/L, 10-7mol/L, and 10-8 mol/L ATRA could inhibit proliferation of H8 cells significantly in a time and dose-dependent manner; (2) According to the observation by Light Microscopy and... |
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