The Inhibitory Effects Of FK228 On Retinal Neovascularization

Objective: To explore the inhibitory effects of FK228 on retinal nevoascularization.Methods: One-week-old C57BL / 6J mice were put into the environment with 75% oxygen for 5 days to establish models of vascular proliferation retinopathy. These mice were divided into normal groups (no treatment), control groups (each mice received an intravitreous injection of DMSO(dimethylsulfoxide)the same volume with treatment groups as a control) and treatment groups(each mice received an intravitreous injection of 250ng of FK228,)was injected into the other eye of the mice both in these two groups ).The ADPase histochemical straining was used for retinal flatmount to observe changes of retinal vessels. counting the endothelial cell nuclei of new vessels extending from retina to vitreous in the tissue-slice. hypoxia-inducible factor-1α(HIF-1α)and vascular endothelial growth factor ( VEGF ) expression were examined by immunohistochemistry. The inhibitory effects of FK228 on retinal nevoascularization were evaluated...