The Study On Preservation Of Lyophilized Red Blood Cells

BACKGROUND:Human RBCs are currently stored at 4℃ in liquid dextrose basedmedia , but the shelf-life is only 35 to 42 days, another method for long-term storage includes treating the cells with glycerol solution and freezing them at -80℃, followded by storage at this temperature for up to 10 years. These two methods require careful maintenance of low temperature during storage and transport in order to preserve the bioligical activity of the erythrocytes. Storage of RBCs in the dry state offers a possibility of storing the cells for long periods of time under conditions that are far easier to maintain at room temperature, making transport to sites of immediate need feasible. So researcher focuse on the preservation of lyophilized erythrocytes.There were thirty years about study of the preservation of lyophilized erythrocytes, but no achievment. Rearcher found the fact that the membrane of RBCs undergo massive fusion and leakage of hemoglobe during freeze-drying. How to prevent fusion of membrane? Rearcher get inspiration from anhydrobiosis. Studies of anhydrobiotic organisms have revealed a complex series of internal physiological and biochemical changes and adaptation that permit these organisms to survive in dried environments, one of change is the accumulation of large amounts of...