Construction Of Mutants Human Hypoxia Inducible Factor-1α And Enhanced Green Fluorescent Protein Recombinant Adenovirus Vectors

IntroductionThe treatments for ischemic heart diease now mainly include the conventional medical treatment, percutaneous coronary intervention(PCI) and coronary artery bypass grafting(CABG). However, a significant proportion of patients have symptoms refractory to medical treatment, and are unsuitable for conventional revascularization therapies. And, in-stent restenosis remains a significant clinical problem with rising incidence of coronary stenting and coronary artery bypass grafting. Obstruction of the arteriole after PCI and CABG is also a severe problem.So it is necessary to find an alternative strategy for the theatment of ischemic heart disease. In recent years, with the progress in molecular biology, gene therapy for angiogenesis in ischemic myocardium has emerged as a novel promising approach in the coronary revascularization. Angiogenesis represents one of the fastest growing fields in biomedical research over the past decade.Among the numerous vascular growth factors, vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF) have been studied deeply. Although the phase I clinical trials have confirmed the safety and efficacy of their gene therapy, the phase II clinical trials,...