| Objective: To study mechanisms and therapeutics of escitalopram on depression.Methods: (1) Thirty male Sprague-Dawley rats were randomly allocated into five groups: normal control group, model group, escitalopram groups (1mg/kg, 2mg/kg, 4mg/kg). All rats except those of normal control group were singly housed and exposed a chronic unpredicted mild stress. The behavior of rats was detected by open - field test and sucrose preference test, and the levels of adrenocorticotropic hormone (ACTH) and cortisol (CORT) in plasma of rats in different groups were measured. (2) The immunohistochemistry method was used to investigate the expression of BDNF and pCREB in hippocampal neurons of rats of each group. (3)Cultured PC12 cells were treated with corticosterone(Cor) 0.2mmol·L-1, at the presence of escitalopram (ESC6.25μmol·L-1, ESC12.5μmol·L-1 and ESC25μmol·L-1).Results: (1)In open-field test, compared with normal control group, squares crossing of rats in model group decreased (82.50±5.822 and 11.00±9.445, respectively). Compared with those of normal control group, rearing of model rats was decreased, which scores were 12.67±3.445 and 1.83±1.835 respectively. Meanwhile, compared with those of normal control group, grooming of model rats was decreased too. Their data were 1.17±0.983 and 3.00±0.894 respectively. Compared with those of normal control group, sucrose consumption preference of model rats decreased in sucrose preference test with data of 86.50±3.782 and 47.83±6.338 respectively. However, compared with those of normal control group, time in centre shelves of model rats was increased and their data were 1.17±0.408 and 3.33±1.033 respectively. The concentration of plasma ACTH and CORT was increased in the model rats,... |
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