The Study On The Ophthalmic Drag Delivery System Of Puerarin

As one of the important fields of controlled release, ophthalmic drug delivery has received extensive interests. The sensitivity and protection mechanisms of the eye promote more effective drug delivery systems with better compliancy to be developed, however, the applications of numerous pharmaceutical methods are limited simultaneously, which presents great challenge in designing ophthalmic dosage forms. In this thesis, Puerarin (PUE) was selected as a model drug. A new controlled-release PUE gel and a new thermosensitive gel PUE were developed using hydrophilic polymers. A series of relative studies were carried out. 1.In the preformation study, two kinds of UV spectrophotometer were developed for assay during the studies of physicochemical properties. And in this part the basic physicochemical properties of PUE were determined. Meanwhile, phase solubility diagrams of PUE/HP-β-CD system was obtained.2. The permeability characteristics of PUE across the cornea of an isolated rabbit were studied in vitro using a variety of permeability enhancers. EDTA showed a significant improvement permeability without toxity to the cornea of an isolated rabbit and could protect PUE by reacting with metal ion. For the above two reasons EDTA was chosen as permeability enhancers of PUE.3. Release behavior of PUE from gel based on various kinds of hydrogel were explored in a dissolution apparatus under stirring conditions. The results showed the PUE release behavior from gel based on 3%K4M and 3%F4M were delayed release. The permeability of PUE gel across isolated rabbit cornea was measured using in vitro method .The apparent permeability of PUE from gel contained 3%K4M showed a improvement as much as 1.35 times, compared with that of in the control(P<0.05) .It indicates that K4M was a better hydrogel base for PUE...