Study On The Anticancer Constituents Of Macleaya Cordata(Willd)R.Br.

In this dissertation, in vitro tumor cells bioassay was used to search anticancer compounds, 16 compounds were obtained from the dry stem of Macleaya cordata (willd)R.Br.. Structures of 14 compounds were elucidated on the basis of chemical evidences and spectroscopic analysis.14 compounds were determined as: Sanguinarine (1), Norsanguinarine (2), Dihydro-sanguinarine (3), 3α-Hydroxy-oleanan-12(13)-ene-30-oic acid (4), N-methyl-4,5-methylene-di-ol-succinimide (5), 6-Acetonyldihydrosanguinarine(6), Oxysanguinarine (7), 6-Methoxy-dihydrochelerythrine (8), Protopine (9), 3,4',6'-Trihydroxy-5-methoxy-2'-methyl-biphenyl-2-carboxylic acid (10), 4-Hydroxy-3-methoxy-cinnamaldehyde (11), 4-Hydroxy- 3-methoxy-benzaldehyde (12), 3,4,5-Trimethoxyphenol (13), β-Sitosterol (14). Compound 2, 4,5, 6, 8, 10 were isolated from M. cordata. (willd)R.Br. for the first time.MTT method was used to test the cytotoxic activity of these compounds on MCF-7, NCI-H460, Hela and HepG2 cancer cell lines as well as human normal 293 cell. Alkaloids(Compound 1 and 8) showed stronger cytotoxic activities in comparison to positive control 10-hydroxycamptothecine, they strongly inhibited the growth of 4 tumor cells together with 293 cell in vitro. Compound 9 showed selective cytotoxicity on Hela and HepG2 cell lines while displayed relatively weak cytotoxicity on human normal 293 cell. Other compounds displayed no cytotoxicity on the five cell lines. These evidences provided that kinds of alkaloids from Macleaya cordata (willd)R.Br. were anticancer constituents. These results also gave some theoretical basis for M. cordata. (willd)R.Br. application in cancer therapy in clinical.