Cholecystokinin Gene -45C/T Polymorphism And The Research Of The Relationship With Depression

IntroductionCholecystokinin ( CCK) gene has polymorphisms, and has a close relationship with some human psychic diseases by influencing or changing the roles of other neurotransmitters on CNS. So, cholecystokinin gene can acts as a new forensic individual identification and parentage testing genetic marker or legal psychiatry testing referential genetic marker.Human CCK gene is mapped to 3ql2. 3. It has 3 exons and 2 introns. And it is about 7 kb. There are two polymorphism loci in the promoter region of the CCK gene, i. e. -196G/A and -45C/T. There hasn't been any report about the allelic frequency of -45C/T polymorphism locus of different populations in our country up to now. Although some achievements have been obtained by foreign researchers, we can not use them directly for the mutation between different races and ethnics. To provide some basic data for forensic application, this stud-y used TaqMan probe technique to detect the genetic polymorphisms of -45C/T locus of our different population groups, and research the genetic association between this locus and depression.Materials and methodsWe use PCR method to amplify the locus, and use TaqMan probes labeled fluorescent dye to hybridize them. Then we detect the genotype of 111 Chinese han and 65 depression patients by sequence detector.