Comparison Of Total Hepatic And Hemihepatic Blood Inflow Occlusion In Rat: A Prospective Randomized Trial

Objective To compare the survival time and rate, pathological changes, the ultrastructure of liver cells , and the serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) between the total hepatic blood inflow occlusion group(TH) and the hemihepatic blood inflow occlusion group(HH) in a rat model.Methods Sixty male SD rats subjected to hepatic blood inflow occlusion were randomly assigned by computer to TH or HH. The two ischemic-reperfusion groups included three different ischemia period: 30minutes, 45minutes and 60minutes with 30minutes reperfusion. After reperfusion, left liver resection was performed for pathologic determination. Liver injury was evaluated using rats survival rate and time, pathologic changes by hematoxylin and eosin(H&E) staining, ultrastructural changes by transmission electron microscope(TEM) , serum levels of ALT and AST elevation between preoperation and postoperative day 1. Results Rats survival rate of postoperative day 7 in HH group was significantly higher than in TH group. After 30 minutes, 45 minutes, 60 minutes of ischemia with 30 minutes reperfusion, HH was associated with better animal survival time compared with TH(P=0.00,P<0.05). The survival...