Prevention Of Chronic Cyclosporin A Nephrotoxicity In Rats With Tranilast

Objective 1.To observe the change of renal function and pathology inchronic cyclosporin A nephrotoxicity rats which were gastric gavaged with tranilast. 2. To investigate preliminarily the changes of expression of renal TGF-β1,OPN,BMP-7 and the number of ED₁ positive macrophages in a rat model of chronic CsA-induced nephropathy when they are treated by tranilast and irbesartan in order to try to elucidate the detailed mechanism of them preventing chronic cyclosporin A nephrotoxicity.Methods After fared 1 week of low sodium diet,69 SD male rats were randomly divided into four groups of eighteen rats(control group have fifteen rats ) as follows. Control group: rats were gastric gavaged of olive oil daily(1.2mg/kg.d); model group: rats received a daily gastric gavaging of CsA (CsA was dissolved in olive oil) 20 mg/kg.d; tranilast therapy group: rats were gastric gavaged of CsA 20 mg/kg.d and tranilast 400 mg/kg.d; irbesartan therapy group: rats were gastric gavaged of CsA 20 mg/kg.d and irbesartan 10 mg/kg.d. The correlated targets were observed at the end of 1, 2 weeks and 4 weeks after gavage of tranilast and irbesartan . The proteins of...