Experimental Study Of Small Interfering RNA Targeting Rac1 Inhibits Retinal Neovascularization In Rat Model

Purpose:Excessive angiogenesis is a critical cause of blindness. Among them retinal neovascularization has a large propotion. Retinal neovascularization and its hemorrhage, exudation and proliferation are major pathologic process in many ocular diseases. Angiogenesis results from many factors. The studies in the past are more focused on a single factor to control angiogenesis. These methods have some inhibitory effects, however they are weak. The recent studies revealed that Rac1 maybe play an important role in modulation of many factors in angiogenesis. In order to reveal the effects of Rac1, In this study ,we apply RNAi to inhibit Rac1 gene. And observe the inhibitory effects on retinal neovascularization.Methods:1. Construction expression vector of Rac1—siRNA1.1 Design Rac1—siRNAon the basis of human's Rac1 mRNA. And make it homologous to rat.