Screening Of Lignicolous Marine Fungi With Antitumor Activity And Secondary Metabolism Compounds Produced By Fungus TY-2

177 strains of lignicolous marine fungi were isolated from Mangrove debris and other submerged wood panels which were collected from the intertidal zone around Xiamen. By using two cell line panels and MTT assay, 24.9%, 44 strains, of all have the antitumor activity. Meanwhile, 6 lignicolous marine fungi were pick out for the research of cultivation assay by different carbon nutrilit. The results show that the distinctions exist in not only the quantity of growth but the antitumor activity of these 6 lignicolous marine fungi cultivated in different media. The products of some strains in cellulose medium have the highest growth quantity and antitumor activity.We classified a lignicolous fungus TY-2 as Phaeosphaeria sp.. In the broth of the strain, employing VLC (Vacuum Liquid Chromatography), Silica Column Chromatography, Dextran gel Column chromatography, Calcium monophosphate Column Chromatography, HPLC and re-crystal, we idolated 10 compounds. 6 identified compounds of this : 1,8-dihydroxy-3-methyl-anthraquinone ,1,3,8-trihydroxy-6-methyl-anthraquinone,1,4,5-trihydroxy-2-methyl-anthra- quinone,3,10-dimethyl-dibenzo[e,g][3,4]-azocin[2,3-b]- dioxin,3-hydroxy- dihydrofuran-2,5-dione and D-mannitol. The others need more data to be identified, but 3 of them can be make certain as anthraquinone.