| Cistanches salsa (C.A.Mey) G.Beck, one species of Genus Cistanche, which belongs to Family Orobanchaceae, is a perennial and parasitic herb growing in Neimenggu, Xinjiang, Gansu, Ningxia and etc. Cistanches includes some active compounds such as phenylethanoid glycosides (PhGs), iridoids and its glycosides, lignans and its glycosides. Cistanches has been used for treatment of improving immune and memory function, anti-oxygenation, anti-radiation, anti-arteriosclerosis, regulating neural and internal secretion, moistening intestines and etc. There are four species and one variety in China, the dried fleshy stems of Cistanche deserticola Y.C.Ma has been specified in Chinese Pharmacopoeia. Due to the decrease of natural resources, some species have been planted and had a good harvest in different area.In this work, through comparing the components of cultivated C.tubulosa (Schenk) R.Wight with natural C.tubulosa, the experimental results showed that the contents of the former were less than the contents of the later in microelement and constituents of amino acid. But the contents of active compounds were almost same in PhGs.A TLC method was established for qualitative analysis of PhGs by using n-butyl alcohol– acetic acid– water (5:4:1) as developing reagents. Aluminii nitras-Colorimeteic method (at 510nm) was established using echinacoside for quantitative analysis of total PhGs in C.tubulosa. The relative standard difference of the method was 0.55%, the recovery ratio was 93.5%～105.1%. And the contents of total PhGs in cultivated and natural C.tubulosa samples were 5.42% and 5.51% respectively by Aluminii nitras-Colorimeteic method. LC-MS was used for quantitative analysis of PhGs monomers.Chromatographic conditions that make PhGs separated completely were the following: column Sunfire C18 (2.1X150mm), methanol– water– 1% acetic acid as mobile phase, flow rate 0.3mL·min-1, temperature 35℃. The contents of echinacoside and acteoside are 3.01%and 1.65% respectively by using LC-MS.PhGs from cultivated C.tubulosa were extracted by using ethanol. The best parameters of technology were optimized using the response surface analysis. Experimental results showed that temperature 60℃, 1:12.8 for the ratio of solid to liquid, 3.75h of extraction time, 68.2% ethanol to whole extraction system, exaction twice. The yield was of 5.73%.Seven kinds of macroporous resins were used to compare their absorption and desorption PhGs from cultivated C.tubulosa. AB-8 resin was determined to be better absorbent material and it possesses high absorption and desorption capacity. AB-8 resin's static and dynamic absorption and desorption experiment showed that AB-8 possessed higher absorption capacity when pH was 3.43, concentration of sample was 3.6～6.0mg·mL-1 and flowing rate was 1.5BV·h-1. Using 40% ethanol as eluent, desorption ratio...
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