Chemopreventive Effects Of Uygur Medicine KuiJieAn On Rat Colon Aberrant Crypt Foci Induced By Dimethylhydrazine

Objective: An investigation was conduced to assess the chemopreven-tive effects of Uygur medicine KuiJieAn, on a rat colon carcinogenesis model induced by dimethylhydrazine (DMH). the result were compared with those of Etiasa. Methods: Fifty-four 8-week-old male Sprague Dawley rats were randomly divided into six groups. Except the normal group, the others were treated with DMH (25mg/kg, subcutaneous injection). Inject repeatedly on the third day. At the first day of injection, the control group and the model group were treated with physiological saline (1ml/100g); The Etiasa group were treated with Etiasa (0.14g/kg); The low dose group was treated with kuijiean (0.3g/kg); The middle dose group was treated with KuiJieAn (0.6g/kgwt); The high dose group was treated with KuiJieAn (1.2g/kg), once a day, respectively for 45 days. The rats weight once every week in experimental. The animal were killed at the end of the experiment (90 days) and the aberrant crypt (AC) and the aberrant crypt foci (ACF) of the colonic mucosa were assessed after being stained with methylene blue. To figure out the thymus index and spleen index. Results: In the model group , the average number of ACF and AC were (141.38±33.46) and (217.00 ± 47.55) respectively. In the normal group, rats no ACF were found. In the Etrasa group average number of ACF and AC were (65.70±14.32) and (127.00 ± 53.53) respectively. In the low dose group, average number of ACF and AC were (86.33 ± 19.63) and (163.33 ± 52.99) respectively. In middle dose group, the average number of ACF and AC were (140.00 ± 43.34) and (215.22 ± 40.45) respectively. In the high dose group , average number of ACF and AC...