Protective Effects Of AFGF On Rats' Renal Tubular Epithelial Cells Damaged By Aminoglycoside Antibiotics In Vitro.

Objective: To study the protective role of aFGF in the injury of GM on cultured renal tubular epithelial cells of rats.Method: After renal cortexes were physically grinded and chemically digested with 0.25% tryspase for about 20 minutes, these primary cells we obtained were cultured with DMEM. Then, the cultured cells were identified by Cytokeratin 18 by immunocytochemistry. The injury model of rats'renal tubular epithelial cells was established by using gentamicin. And the protective role of aFGF in the injured renal tubular epithelial cells was observed by using some biochemical and enzymological indexes.Result: By observing the morphology of the cultured cells and identifying the cells with Cytokeratin18 , comparing GM group with control group, SOD,CAT, Na+—K+—ATP enzyme activity are decreasing , NAG activity and MDA quantity are increasing (P<0.01) in GM group, demonstrating that the culture of reanal tubular epithelial cells and the establishment of injured model are successful. The differences of the content of biochemistry and enzymology between GM group and aFGF+GM group are significant (P<0.01). However the differences of CAT activity, SOD,Na+—K+—ATP enzyme activity and NAG activity between aFGF+GM group and control group are insignificant ( P > 0.05), but the change of MDA and the activity of SOD is still significant ( P <0.05).Conclusion: aFGF could protect the injurious renal tubular epithelial cell in some degree.