Rapid Detection Of Mycobacterium Tuberculosis In Paraffin-embedded Tissues By Membrane-based Gene Chips

Background and Objective Tuberculosis is one of infectious diseases and the main threats for mankind. Tuberculosis is becoming more and more critical since the emergence and spread of AIDS and multidrug resistant (MDR) strains of M.tuberculosis. Conventional techniques, such as clinical features, histopathology, demonstration of acid fast bacilli (AFB) and isolation of Mycobacterium tuberculosis from the clinical specimens, are limited for diagnosis of tuberculosis in clinically because of their speed, sensitivity and specificity. Gene chips are demonstrably highly sensitive and specific but expensive. To develop a novel diagnostic method for tuberculosis, membrane-based gene chip, a newly developed molecular biological technique, was used for rapid detection of Mycobacterium tuberculosis.Methods (1) Acid-fast staining was used to detect the presence of acid fast bacillus in 120 paraffin-embedded tissues which were clinically and histologically suspected tuberculosis infection. (2) PCR technique was used to detect the IS6110 specific insertion sequence DNA of Mycobacterium tuberculosis complex in the 120 paraffin-embedded tissues. (3) Membrane-based gene chip technique was used to detect the Mycobacterium tuberculosis in the 120 paraffin-embedded tissues. (4) Twenty seven paraffin-embedded tissues of non-tuberculosis were used for negative controls.Result 1. Histological observation:(1) HE staining: Microscopically, all samples showed epithelioid granulomas, which were composed of epithelioid macrophages, Langhans giant cells, lymphocytes, a few fibroblasts with collagen, and characteristic caseous necrosis...