The Effects Of FRNK On Proliferation And Apoptosis Of HSC And Their Molecular Mechanisms

The activation, proliferation, adhesion as well as migration of hepatic stellate cell (HSC) are the central events in the liver fibrogenesis. The cell behaviors are regulated by many signal molecules in HSC.Focal adhesion kinase (FAK) is a critical mediator that connects integrin and the downstream signal molecules in integrin-signal transduction pathway, and is the convergence of many signal pathways. FAK activation, demonstrated by an increase in phosphorylation of Tyr³⁹⁷ as well as other sites in the protein, can recruit and activate the downstream signal molecules. Activation of FAK results in promotion cytoskeleton reorganization and the expression of some genes, which regulate many cellular behaviors. Ras-dependent mitogen-activated protein kinase (MAPK) pathway is one of the clearest signal pathways. Extracellular signal-regulated kinase_1/2(ERK_1/2) is a member of MAPK family. The Ras-Raf-MEK-ERK pathway is involved in many cellular behaviors, such as cell spreading, cell migration, proliferation, differentiation and apoptosis.The C-terminal, non-catalytic domains of FAK, termed FRNK (FAK-related-non-kinase), is expressed independently in certain cells. It is known to all that FRNK is as negative...