Expression And Biological Role Of LPA Receptors In Patients With Epithelial Ovarian Tumors

Objective: Lysophosphatidic acid(LPA) is the simplest glycerophosphilipid, which consists of three structural domains: a phosphate moiety, a linker region and a lipophilic tail. As the cellular lipid mediator, LPA mediates a broad range of cellular responses: smoomth musscle cells contraction, modulation of chemotaxis, neurite retruction, platelet aggrogation, regulation of cell proliferation and transcelllar migration etc. In 1995 Xu found a dramatic elevation of LPA levels in serum and ascite with ovarian cancer, thinking that LPA maybe the activatining factor of ovarian cancer. Westermann compared the levels of LPA in different cancers founding that LPA is higher in ovarian cancer than the others. Those suggests LPA has a positive role in the carcinogenesis and development of ovarian cancer. With the development of these studies, we found LPA bring about its effect by binding to an extracellular receptor. They are called G-protein coupled receptors. LPA has been shown to interact closely with three receptors, LPA₁/Edg₂,LPA₂/Edg₄ and LPA3/Edg7. Through detecting the LPA receptors ,we can make the relation of LPA and ovarian cancer clear and look for a new curative target. So we use PT-PCR and Western-Blot to detect the expression of LPA receptor in normal ovarian, benign...