Experimental Study Of Gene Therapy On Salivary Adenoid Cystic Carcinoma

Objective: Salivary adenoid cystic carcinoma (SACC) is a very common malignancy of salivary gland. Recurrence and early metastasis are its biological properties. It is difficult to be entirely resected surgically and is invalid to radiotherapy and chemotherapy. So the long-term prognosis of patients is very dismal. Therefore, it is imperative to find a perfect auxiliary therapeutic treatment. As a new method for tumor therapy, gene therapy has a large development. Herpes simplex virus thymidine kinase/ganciclovir (HSV-TK/GCV) method is one of the most frequently utilized measures of gene therapy. However, suicide gene therapy alone cannot completely eliminate tumor cells, so it is necessary to combine suicide gene therapy with other therapy to improve performance. This study is to observe the depressing kill effect on SACC animal model in nude mice by union treated with HSV-TK and IL-2 adenovirus vector.Materials and Methods: 1 Materials: (1) Cell line:a human adenoid cystic carcinoma cell line with high tendency of lung metastasi(sACC-M); (2) Drug: adenovirus vector HSV-TK, adenovirus vector IL-2, ganciclovir (GCV); (3) Animal: Twenty SPF grade, 4~6 week-old female Balb/c nude mice were used in this study. 2 Methods: (1) animal model: ACC-M cells were...