Study Of Static Magnetic Fields (SMF) With Anticancer Drug On Tumor

Aim: The synergistic anticancer effects of magnetic fields with anticancer drug on cancer cells were studied. We reestablished B16 melanoma model hi mice and studied effects of static magnetic fields on B16 melanoma.Methods: 1. The influence of SMF or/and anticancer drugs (Taxol) on K562 cells were detected by MTT test. 2. The morphologic changes of K562 cells exposed, under SMF with anticancer drug were observed by microscopy, atomic force microscopy (AFM); The degree of DNA damage and the cell cycle distribution of K562 cells exposed under anticancer drugs or/and SMF were measured by Single cell gel electrophoresis (SCGE) and flow cytometer (FCM) . 3. The influence of SMF or/and adriamycin on B16 melanoma cells were detected by MTT test. 4. B16 melanoma model in mice were reestablished. And the effects of static magnetic fields on B16 melanoma were studied.Results: 1. When cell desity was l×l0~5cells/mL, SMF can enhance the anti-cancer effect of taxol after exposing under SMF and taxol (10ng/mL) for 24h.2. When K562 cells were treated with SMF and taxol for 24h, there were some changes in the morphology of K562 cells, such as some protuberances and holes-like structures appeared on the cell membrane; the degree of DNA damage were enhanced, and the cell cycle distributions were changed. K562 cells mostly were blocked S and G2/M phase.3. When cell desity was 5×10~4cells/mL, the growth of K562 cells were inhibited after exposing under SMF for 24h (p<0.01); When B16 cells were treated with SMF and adriamycin (2ng/mL) for 24h, the cell activities were significantly reduced( P＜0.01) and the degree of DNA damage were enhanced (p<0.01) .4. To study the effects of different number of tumor cells on tumor growth in mice. Different numbers of tumor cells were injected subcutaneously at the right armpit of...