Differentially Expressed Genes Related To Human Gastric Cancer MGC803 Cells Induced By Diallyl Disulfide

Objective: To isolate and cloning differentially expressed genes in human gastriccancer MGC803 cells induced by diallyl disulfide (DADS), explore the related molecular mechanisms.Methods: Cell morphology was observed by inversion microscope and optics microscope M GC803 cells. Inhibition of MGC803 cell proliferation was shown by MTT assay. The activities of alkaline phosphatase (ALP) were detected by ALP assay. Differentially expressed genes induced by DADS on human gastric cancer MGC803 cell line were determined by using suppression subtractive hybridization (SSH), T/A cloning, sequenced and analyzed for homology in the Genbank database with BLAST. Results: Morphometric change show when 30mg/L DADS was added to culturemedium 24h after cell seeding, this agent was found to reduce the number of adherent cells in the culture flask compared with cells cultured in the presence of vehicle alone. MGC803 cells that still adhere the flask showed malignance declined as nuclear-to-cytoplasmic ratio was reduced, the number of nucleoli in the cell nuclear decreased, and coloring of nucleus turned weak.MTT assay showed that apparently proliferation inhibition in MGC803 cell line treated with DADS could be seen and exhibited a dose-dependent model.The concentration of 10, 20, 30,40, 50mg/L DADS for 2 4h suppressed MGC803 cells proliferation by 15.2%, 26.2%, 51.4%, 57.3%, 70.0%.ALP specific activity detection showed that DADS can distinguished diminish the ALP activity and down regulated ratio depending on treatment doses. Adding 10, 20, 30, 40, 50mg/L DADS for 24 h made the ALP specific activity decreasing ratio reached 13.7%, 25.6%, 49.9%, 66.5%, 72.0%。...