Effect Of The Proliferation Of Breast Cancer Cell Induced By ASODN And Its Influence Mechanism

Objective: To explore the inhibitory effect of the antisense ODN of hTERT for the growth of breast cancer cell lines, and to investigate the change of apoptosis, cell cycle and bcl-2 protein expression, then discussing the mechanism.Methods: In the present study, we have investigated the effect of ASODN on humanbreast cancer cells MCF-7 in vitro. Using lipofectamine-mediated DNA transfection technique the antisese telomerase RNA gene phosphorothioate oligodeoxynucleotide was introduced into MCF-7 breast cancer cells, the changes of telomerase activity were detected by Polymerase chain reaction- enzyme linked immunoassay(PCR-ELISA) method. MTT method and flow cytometry (FCM) were used to observe the proliferation,apoptosis and cell cycle of the transfected MCF-7 cells. The expression of Bcl-2 protein was detected by S-P immunohistochemical method.Results:1. The antisense telomerase RNA gene phosphorothioate oligodeoxynucleotide was successfully introduced into MCF-7 cells, which could remarkably inhibit the proliferation of the MCF-7 cells, with the rise of ASODN concentration and for more time as shown in Table 1 and 2, and the different time and different dose's Absorbency value also have the discrepancy (P < 0.01), while SODN group, control group and Lipofectamine group have not the discrepancy(P > 0.05).2. The experiment of flat cell colony form demonstrate that ASODN could inhibit the form of the cell colony, the rate of inhibition was 66.6%. But SODN have no effect on the cell colony form compared with control group and Lipofectamine group.3. The telomerase activity of human breast cancer cell MCF-7 decreased with the rise of ASODN concentration and for more time as shown in Table 4 and 5, and the different...