Study On Separation, Purification Of Bio-active Polysaccharides In A Chinese Herbal Medicine-Compound Immunoenhancer

Because it's avidity on antitumour, antivirus, anticaducity, antiinflammation, anticruor and decreasing blood sugar et al, polysaccharide has become a hot field of scientific studies. In this paper, polysaccharides in Compound Immunoenhancer were extracted and separation was carried out towards active polysaccharides. Three polysaccharides were obtained with their relative molecular weights (Mr) and their monosaccharide compositions confirmed.1.The polysaccharides in Compound Immunoenhancer were extracted adopting ultrasonic-assisted extraction method and content determination was done applying the method of phenol-sulphate acid. Results of content determination showed that the calibration curve of polysaccharide was linear with absorbance in the range from 24.2μg·mL-1 to 96.8μg·mL-1, with R= 0.9994. This method was next used to measure the content of polysaccharide in Compound Immunoenhancer, which proved that the content of the extract was 42.82%.2.Three polysaccharides, PS1-A-Ⅰ, PS2-A-Ⅱa nd PS3-A-Ⅲ,were obtained adopting ethanol-precipitating, DEAE-Cellulose ion-exchange chromatography and Sephadex G-200 gel chromatography.3.Three polysaccharides were proved homogeneous by TOYOPEARL HW-55F gel chromatography, paper chromatography and ultraviolet spectrophotometry. And no nucleic acid and protein were found.4 . The relative molecular weights of three homogeneous polysaccharides were determined 389941, 124451, and 7586 by TOYOPEARL HW-55F gel chromatography. The monosaccharide composing three polysaccharides were also investigated: PS1-A-Ⅰwas composed of one kind of sugar——glucose, PS2-A-Ⅱof galactose, mannose and arabinose, and PS3-A-Ⅲof two kinds——galactose and arabinose.Three polysaccharides got in this study provided support for investigation on immune mechanism of this Chinese herbal medicine. At the same time, this paper would promote the utilization of officinal resource in Xinjiang and studies on traditional Chinese medicine which enhances immune system, and it's sure to be helpful to researches on the mechanism of Compound immunoenhancer.