The Bioeffects Of Silica Nanoparticles On Cells

With the fast development of nanotechnology, the application of nanomaterials in biomedicine has been to a new phase. So the bioeffects of the nanomaterials have gradually become a new focus in the research fields flowing the research and exploitation of nanomaterials. Recently, silica nanoparticles as one of the important nanomaterials display several features of straightforward synthesis and easily modification, which make them be widely applied in biomedicine. However, the bioeffects of silica shell nanoparticles have not been systemicly studied. This thesis is aimed at the cell bioeffects of silica shell nanoparticles. A series of studies on cell bioeffects of silica nanoparticles have been operated. And a kind of muli-groups modified silica nanoparticles with good stability and also easy modification has been developed. This thesis is composed of the following three parts:1. The bioeffects of RuBpy silica nanoparticles on COS-7,EVC304 and L02 cells have been studied by use of different evaluation methods. The results indicated that no measurable cytotoxic effects of nanoparticles being observed on the growth and proliferation of the three kinds of cell lines at a certain range of nanoparticles concentration. Moreover, cells still remained good shape and growth state after the uptake of nanoparticels.2. In order to probe the effect of silica nanoparticles on environment or human, the bioeffects of silica nanoparticles (SiNP), phosphorylate-terminated nanoparticles (PO₄NP) and amino-terminated nanoparticles (NH₂NP) on HaCat cell line has been investigated. Results indicated that the bioeffects of the three kinds of nanoparticles on HaCaT cells were concentration-dependent, and the following order was observed: NH₂NP> SiNP> PO₄NP. In addition, the quantity and rapidity of cellular uptake of nanoparticles by HaCaT cells were diverse due to the different functional groups. Under the same conditions, NH₂NP was most and fast internalized by HaCaT cells, followed by SiNP, and PO₄NP was the least and slowest.3. We studied the surface modification and stability of silica nanoparticles based on the two front parts of work. A simple method that can be more readily conjugate different function groups to the nanoparticles surface was obtained. And a kind of muli-groups modified silica nanoparticles with maximal stability and easy modification by conjugated an optimum proportional phosphate groups and amino groups has been developed, which provided a new chance for the application of silica...