| AIM:With the improvement of people's living conditions, the changes of spectrum of disease, the ageing of the population, ischemic stroke is considered to be one of the major diseases that significantly impair quality of life. And hydroxysafflor yellow A is the main active compound that was extracted from the flower of the safflower plant, Carthamus tinctorius, which is one of the traditional Chinese medicine. This study is designed to investigate the effects of HSYA on angiogenesis after focal cerebral ischemia and its primary mechanism.METHODS:The establishment of focal cerebral ischemia in rat described by Zea Longa, et al, whereby a monofilament suture is inserted into the lumen of the internal carotid artery to block the origin of the middle cerebral artery is a widely used method of modeling focal cerebral ischemia. A total of 27 healthy adult male Sprague-Dawley ( SD ) rats, weighting from 260g to 280g, were randomly divided into three groups, with three animals in each group, as follows: The sham-operated groups, the model groups, HYSA groups (HSYA 48mg/kg via sublingular vein injection at 30 minutes point after the onset of middle cerebral artery occlusion, MCAO). At 24, 72, 144 hours after onset of MCA occlusion, these rats were anesthetized again and the brains were removed, and the 10% paraformaldhyde was perfused through the aorta. The brain was cut in the coronal planes at the intervals 2mm from the front globe and the samples were handled with conventional methods. The changes were investigated by the pathological examination with the methods of HE and immunohistochemistry staining. Immunohistochemistry analysis was performed to ensure the selective detection of immunoreactive factor VIII related antigen (Fâ…§-RAg) , vascular endothelial growth factor (VEGF) and hypoxia induced factor-1 alpha (HIF-1 alpha) with an anti-body. Morphological changes of immunohistochemical VEGF and HIF-1 alpha expression in cortex were compared at different time points after MCA...
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