In Vitro Regulatory Effects Of Serum From Severe Trauma Patients On NF-κB And Their Relationships With Organ Dysfunction

Organ dysfunction (OD) and multiple organ dysfunction syndrome (MODS) are one of the major complications and important death cause of trauma, and the mortality is very high in clinic because of lacking special and effective therapeutic strategies. Though the treatments for prevention and cure of MODS are always being developing, no great progress has been made in decreasing incidence and mortality of MODS. So estimating the occurrence of OD and MODS and taking necessary treatments in early phase of OD and MODS may decrease complications and mortality of severe trauma patients. In our study, luciferase (LUC) reporter gene expriments are used to observe in vitro regulatory effects of serum from severe trauma patients on NF-κB, and to investigate the effects of the serum plus LPS on the activity of NF-κB in macrophages. In addition,relative cytokines were detected to predict the occurrence of OD and MODS.Objective To investigate in vitro regulatory effects of serum from severe trauma patients on NF-κB, changes of TNF-αand IL-10 concentrations and their relationships with OD and mortality.Methods The recombinated NF-κB plasmids containing LUC reporter gene were transfected into the macrophages of mice (RAW264.7), then the macrophages were stimulated with serum from 20 healthy people and serum from 26 severe trauma patients in the presence or absence of LPS. The activity of LUC reporter gene was analyzed with the detection kit. The concentrations of TNF-αand IL-10 in serum were measured by enzyme-linked immunoadsorbent assay (ELISA). Injury Severity Scores (ISS), Acute Physiology and Chronic Health EvaluationⅡ(APACHEⅡ) and the occurrence of OD were recorded. Changes of NF-κB activity and concentration of TNF-αand IL-10 were analysed statistically.Results...