Experimental Study Of The Effects On Neuron By Neurotrophic Factors After Hypothermia Cardiopulmonary Bypass

With the development of intracardiac operation under direct vision and postoperative care, there has been a great advance in the therapy for severe and complicated congenital heart diseases and aortic malformation. The technique of deep hypothermic circulatory arrest (DHCA) has been used widely in these operation. DHCA will not only supply clear visual field but also cut down the metabolism of brain and protect brain. However, when the operation time surpass the safe time limit (45 min), it will lead to the shortage of energy and accumulation of metabolitic product, aggravate brain damage, and bring about many postoperative complications of nervous system .Neurotrophic factors (NTF) are special protein and peptide, and can . promote regeneration of neuron, repair damaged neuron, inhibit excitatory amino acids, reduce intracellular calcium overload and apoptosis, enhance antioxidase activity, and relieve lesion of free radicle. NTF include: 1. NTF family: nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), NTF-3~6 (NT-3~6), glial cell line-derived neurotrophic factor (GDNF), ciliary neurotrophic factor (CNTF). 2. Basic fibroblast growth factor (b-FGF).