A Study On The Protective Effect Of Aged Mice Complicated With Tuberculosis By Mycobacterium Vaccae

Purpose: To explore the protective effect of mycobacterium vaccine to pulmonary tuberculosis of the experimental aged mice. Methods: 30 aged C57BL/6J mice were randomly divided into three groups: the non-immunity protective group (group C); the mycobacterium vaccae preventative group (group M), and the Bacillus Calmette-Guerin (BCG) protective group (group B) . All the subjects were injected subcutaneously 0.1ml Physiological saline, 22.5μg mycobacterium vaccine, and 0.1 mg BCG respectively at the 0 week. Four weeks later, 0.2ml standard vaccine of H₃₇R_v with concentration of 5 × 10⁵/ml was injected through the lateral tail vein to attack the toxin. Six weeks later after the infection, the mice were sacrificed. The fluorescence-activated cell sorter was used to test the Peripheral blood T lymphocyte sub-groups of the mice, evaluate index of tubercular lesion and to count the numbers of mycobacterium tuberculosis in the alveolus macrophages, quantitative culture of mycobacterium tuberculosis of lung...