The Mechanism Of Apoptosis In K562 Cells Induced By Berbamine And STI571 Alone Or In Combination

Chronic myelogenous leukemia (CML) is a myeloproliferative disorder characterized by the presence of the Philadelphia chromosome(Ph), t(9:22),which expresses bcr/abl oncogene. It is generally believed that CML develops when a single, pluripotential, hematopoietic stem cell acquires a Ph chromosome carrying the BCR-ABL fusion gene, which confers on its progeny a proliferative advantage over normal hematopoietic elements and thus allows the Ph-positive clone gradually to displace residual normal hematopoiesis.Cysteinyl aspartate-specific proteases (caspase) are correlated with maturation of cell factors and apoptosis, which showed a important role in singal transduction of apoptosis .Caspase-3 is the key in the pathway of apoptosis, its activation induces apoptosis,while the anti-apoptosis by bcr/abl in CML is through inhibiting the expression of caspase-3. Survivin is an inhibitor of apoptosis protein(IAP) and is regarded as the most powerful inhibitor of apoptosis. Survivin is closely correlated with the apoptosis and cell cycle modulation. It was demonstrated that Survivin selectively expressed in commonly seen tumors, which included malignant hematopoietic tumor, and it was closely correlated with the clinical therapy and prognosis of leukemia patients . Studies have showed survivin abrogated the...