Inhibition Of NRAGE Gene Expression In Gastric Cancer SGC7901 Cells By ShRNA

RNA interference (RNAi) is the process of sequence-specific posttranscriptional gene silencing (PTGS) triggered by double stranded RNA (dsRNA). The dsRNA introduced or generated in cells is subjected to digestion with a dsRNA-specific endonuclease, Dicer, into 21-23 nucleotide (nt) RNA duplexes, and the resultant duplexes, referred to as short interfering RNAs (siRNAs) duplexes, function as essential sequence specific mediators of RNAi in the RNA-induced silencing complexes (RISC). These reactions result in the cleavage of the target mRNA. As one form of RNAi, short hairpin RNA (shRNA) is composed of both sense and antisense sequences with a few bases (19-21nt) which are reverse repeat sequences named as loop between them. Since discovered in 1998, RNAi has been successfully used for many human tumors study, including colon cancer , oesophagus cancer, liver cancer, ovary cancer et al. Little research in the field of gastric cancer using this technique has been reported yet.NRAGE(neurotrophin receptor-interacting MAGE homoloue)which was found in 1999 is a new member of the MAGE-D subfamily of MAGE genes. The rat homolog of MAGE-D1 was named NRAGE , but the mouse homolog of MAGE-D1 was named Dlxin-1. NRAGE express in embronic tissues , tumorous tissues and nomal tissues other than other genes of MAGE family express only in embronic tissues and tumorous tissues. NRAGE gene interacts with p75...