Protective Effects And Mechanisms Of The N-butanol Extract Of Morinda Officinalis How On Neonatal Rat Cardiomyocytes By Hypoxia/reoxygenation Injury

It is vital for patients to restore blood supply in the early time of AMI. With the study on myocardial ischemia/reperfusion injury(MRI), people find that MRJ has become more and more severe myocardial injury at the same time of getting coronary artery opening again. Therefore, alleviating myocardial ischemia/reperfusion injury has been one hotspot in the clinical and basic study of cardiology. Recently, there has been great development in Chinese medicine to protect myocardium against ischemia reperfusion injury. Some of them have been used in clinical treatment of myocardial ischemic disease. The extract of morinda officinalis How (MOH) has been reported to increase antioxidants in brain and liver. Previous study has demonstrated that the aqueous extract of MOH has evidently protective effects on MIRI induced by ligation of coronary artery, and the ethanolic extract of MOH has also protective effects but there are no reports so far about its mechanisms in cultured neonatal rat cardiomyocytes. The purpose of this study is to investigate the effects and its mechanisms of the n-butanol extract of MOH(BEM) on hypoxia/reoxygenation injury in cultured neonatal rat cardiomyocytes. MethodsThree different concentrations of the n-butanol extract of MOH(BEM): 0.038g·L^-1, 0.11g·L^-1 and 0.34g·L^-1 were prepared. The hearts were isolated from...