The Research About Interference Of Nucleostemin-Specific Short Hairpin RNA In Vitro And In Vivo

Objective: Nucleostemin (NS) is a novel protein found in the nucleoli of stem cells and cancer cell lines. Nucleostemin is abundantly expressed while the cells are proliferating in an early multipotential state; however, when cells differentiate, nucleostemin expression decreases rapidly and remarkably prior to cell-cycle exit. Nucleostemin plays a role in maintaining self-renewal of stem cells and cancer cells. Depletion or overexpression of nucleostemin reduces cell proliferation in stem cells or transformed cells. In the hematopoietic system, nucleostemin gene is found preferentially expressed in the c-kit~+/lin~- adult bone marrow population but not in granulocytes and lineage-committed B lymphocytes. Among hematologic malignancies, acute myelogenous leukemia is typically a disease of stem cell in origin. The HL-60 and K562 leukemic cells showed a high level of NS gene expression by RT-PCR technology. RNA interference is one of the most important mechanisms of post-transcriptional gene silencing (PTGS). Small interference RNA (siRNA) can knockdown the expression of specific gene effectively. When input into cells, short hairpin RNA (shRNA) can be cleavaged into siRNAs by Dicer. ShRNA can keep high...