Computer Assisted Design And Application Of Electrophoresis Microchip Of Clinical Assay Of Serum Digoxin

This work concerned to combine the highly integration, powerful separation, and skillful fluid manipulation of electrophoresis microchip with the specifity of immunoassay and the high sensitivity of chemiluminescence detection to develop a clinical detection method of serum digoxin based on microfluidic chips. Mathematical model of electrophoresis on microchip was taken to calculate and simulate the electric and flow field distributions in microchannel,which was validated by experimens. Electrophoresis and fluorescence photograming experiments on chip were conducted to investigate the effects of chemical reaction, reagents adsorption, and solutes volatilization on electrophoresis process, of which the results could not be obtained through simulation only. Combining the computer simulation with relative experiments, the computer assisted chip design procedure based on sample properties and detection principle was established. Focusing on the cardiac glycoside digoxin which has narrow therapeutic range and requires frequent monitoring, the chip applied to chemiluminescent enzyme immunoassay of serum digoxin was designed by the established design approach. The pre-column enzyme immuno reaction- electrophoresis separation- post-column chemiluminescence detection method of digoxin was developed, with the expectation to apply to clinical assay of digoxin. The research details and relative conclusions were inlcluding:ConventorWare, was employed to simulate the passive mixing processes within micro channels of different dimensions and structures. The simulation results were compared with the experimental images of Rhodamine 6G mixing process in glass-PDMS micro channels. According to the fluidic and electric fields distributions, mixing effects of laminar flow in micro channels of different geometric pattern were compared and discussed basing on the calculated mixing efficiency. The mixing process of Rhodamine 6G with buffer was performed and its mixing efficiency was calculated using MATLAB. In terms of the mixing efficiency versus mixing length curves, the contribution of different dimensional turns to mixing capability was quantified and validated. Furthermore, a new electro kinetic multiple laminar mixer with high performance was presented and validated by simulation and experiments, which only needed simpler fabrication and operation compared to the traditional one, which was applied to the pre-column immuno reactor of digoxin microchip.