| Hepatoma-derived growth factor (HDGF) was isolated from hepatoma cell line HuH-7 could promote the growth of a variety of cell lines. HDGF represents a new family of growth factors called HDGF-related proteins (HRPs), encompassing HDGF, HRP 1,2, 3, 4 and lens epithelium-derived growth factor (LEDGF)/p75/p52. All of these factors contain a well-conserved N-terminal amino acid sequence (homologous to amino terminus of HDGF; HATH) and have two putative bipartite nuclear localization signals (NLSs) but lack a signal peptide-like hydrophobic sequence essential for classic secretion.Hepatoma-derived growth factor related protein 2 (HRP-2 ), which belongs to the family of hepatoma-derived growth factor (HDGF), mapped to region 19pl3.3, be composed of 12 exons and encodes a 2016bp cDNA, which upon transcription yields, consisting of 671 amino acid,. The similarity between HRP-2 and HDGF is 12.7%. As typical for members of the HDGF family, HRP-2 is characterized by the HATH domain.The high homolog indicate the domain is very important to the bio function of the family. The core structure of the HATH domain is a five-stranded P-barrel followed by two a-helices, similar to those pwwp domains of known structures. The pwwp domain, which was named after the relatively well-conserved residues, Pro-Trp-Trp-Pro, is composed of ～90 residues and is present within all eukaryotes, pwwp domain are involved in the process of transcriptional regulation, DNA repair, and DNA methylation. Surface Plasmon responance results showed that the HATH domain was primarily responsible for heparin binding.In this paper, we describes for the first time the human HRP-2, which was ubiquitously expressed in the almost all tissues, but highly expressed in the testis. As the function is dependant on its structure, to investigate the importance of HATH (pwwp) domain to the function of HRP-2, we tried to find relationship between HATH domain...
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