Ananlysis Of Methylation Profiles Of APC Gene Promotor With Newly Developed Methylation-specific Microarray On Lung Cancer

BackgroundIt is now clear that aberrant methylation of CpG islandstretches within a promoter, causing silencing of tumor suppressor genes, is a widespread phenomenon in cancer cells.It may be a reliable diagnostic and prognostic marker of early stage lung cancer. APC gene is a tumorsuppressor gene. DNA hyper-methylation in the promoter region of APC gene increases mutation rate in exons and silence the gene so that it loses control of cellular proliferation. At present, a variety of methods have been used to evaluate the methylation status of genes: Southern blot, bisulphate genomic DNA sequencing, restriction enzyme-PCR, methylation-specific PCR (MS-PCR), methylation-sensitive single nucleotide primer extension (MS-SNuPE), denaturing high-performance liquid chromatography (DHPLC) and electrochemistry.But these methods only can ananlyse limited number of CpG islands . Accordingly, they do not fulfill the need to quantify the level of methylation relating to the gene silencing.The microarray of methylation solves the question. ObjectiveTo develop a methylation-specific microarray(MSM) for analyzing...