Expression And Clinical Significance Of SEL1LmRNA And Protein, Smad4 Protein In Esophageal Cancer

Objective To investigate the expression of SEL1L mRNA and protein, Smad4 protein in esophageal cancer and their relationship with clinical pathological features. To explore their roles in carcinogenesis and progression of esophageal cancer and to evaluate their clinicopathologic significances. Methods Immunohistochemical staining (S-P method) for SEL1L protein and Smad4 protein were performed in 90 esophageal carcinoma, 35 normal esophageal tissues 5cm distant from tumors, 60 esophageal mucosa adjacent to tumors and 20 esophageal dysplasia. In situ hybridization(ISH) for SEL1L mRNA was examined in 90 esophageal carcinoma, 35 normal esophageal mucosa 5cm distant from tumors, 60 esophageal mucosa adjacent to tumors. Results 1. The positive rate of SEL1L mRNA was higher in esophageal carcinoma (80.0%), compared to normal esophageal mucosa (14.3%) and esophageal mucosa adjacent to tumors (16.7%) . The difference was significant (P<0.01). The positive rate of SEL1L mRNA in the tissue of with lymph node metastasis (92.7%) was higher than that of without lymph node metastasis (69.4%) (P<0.01) . 2. The positive rate of SEL1L protein was higher in esophageal carcinoma (87.8%), esophageal dysplasia (90.0%) , compared to normal esophageal mucosa (14.3%) and esophageal mucosa adjacent to tumors (13.3%) respectively. The difference was significant (P<0.01). The expression of SEL1L protein in esophageal squamous cell cancer was not related to any clinicopathological parameters such as gender, age, tumor location, tumor size, degree of differentiation, depth of tumor invasion, lymph node metastasis and clinical stage (P>0. 05) . 3. The positive rate of Smad4 protein was...