Screening And Identification Of Human Fab Antibody Against Idiotypic Antibody Of Schistosoma Japonicum From Large Naive Phage-display Library

The schistosomiasis disease caused social and economic hardship, and the rates of morbidity and mortality were high.Significant progress has been made though drug thereapy in the control of schistosomiasis in China. However, re- cent data suggest that the disease is re-emerging. As an important approach to the preven- tion and treatment of schistosomiasis, schistosomiasis vaccines have aroused great attention. At present, work is focused on finding a vaccine for schistosomiasis by using genetic engineering.As the use of sugar materials or molecules containing sugar (proteoglycans, glucoproteins and glucolipides) are the protective antigens of schistosoma,it is difficult to produce vaccines by the technique of genetic engineering. It is now hoped that the problem can be solvedby using anti-idiotypic antibody (anti-id) techniques. The hybridoma cell of anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum was already established by the Guan xiaohong professor. NP30 can induce protection immunity of certain degrees after mouse and goat in the experimental groups were actively immunized with NP30.But the NP30 is an antibody estibished from mouse.As an heterogeneity protein, it may cause human anti-mouse antibody (HAMA), which restrict in human application. This article is using phage display technology constuncted a naive library of phage-display human Fab library.Obtained the completely humanized Fab antibody against idiotypic antibody of schistosoma japonicum from large naive phage-display library.It may useful to study on anti-idiotypic antibody...