Devolopment Of Mouse Tooth Germ Heterotopically Transplantation In The Nude Mouse Muscle

Whether in the clinic field or in the preclinical medicine field, the regeneration of periodontal tissue is more and more attached importance to. Because the mechanism of tooth development in late stage is very complex, it can't be simulated in vitro. The animal model at the present time only was indicated for tooth germs development in early time. There are many limitations in study the development of tooth root and the formation of the periodontal tissue in the late stage of tooth development in these models. So the specific mechanism of the regeneration of periodontal tissue is unknown today. The dental follicle plays an important role in tooth development. Most study of dental follicle is about its role in forming periodontal tissue and in tooth eruption. Almost no study is about its effect on tooth root development. In this study, an innovative experimental model will be established for research the tooth development in late stage. At the same time, the role of dental follicle in the root development is determined.ObjectiveThe changes of the developing mouse's dental germs after transplanting into nude mouse was observed in order to study whether this innovative experimental model can be used in the study of late development of mouse dental germs. At the same time, the role of dental follicle in tooth development was determined, so that the mechanism of periodontal regeneration was explored.Methods(1)Sixteen mandibular first molar dental germs from eight five-day postnatal Balb/c mice were divided into two groups. Dental follicle of the first group was undetached, dental follicle of the second group was removed. Subsequently, each of the dental germs was separately supermucule-transplanted to adult nude mice. At the...