Coculture With Granulose Cells Of Human Oocytes During In Vitro Maturation And The Influence On The Maturation Of Ooplasm

Objective IVM technique plays an important role in assisted reproductive technologies, especially for PCOS patients. The current in vitro culture medium can achieve nuclear maturation of oocytes rather than cytoplasmic maturation. This study is designed: firstly, to identify whether nuclear and cytoplasmic maturation can reach simultaneously in vitro maturation. Secondly, to observe if in vitro culture time can affect nuclear and cytoplasmic maturity of oocytes . Thirdly, Many animal experiments have identified that cocultured with monolayer granulose cells(GCs) can greatly improve the ooplasmic maturation, which is manifested in high fertilization rate, high embryo quality and high pregnancy rate. Our objectives were to confirm that cocultured with GCs can surely improve ooplasmic maturation of human oocytes. In this experiment, we used the linear distribution of cortical granules(CG) as the marker of cytoplasmic maturity of oocytes, since many reports have defined that. In addition to that, in the clinic, the statistical rates of maturated oocytes,fertilized oocytes after ICSI,high quality embryoes can also tell the maturality of cytoplasm.Materials and Methods (l)source of immature oocytes:Immature oocytes were retrieved from PCOS patients who were undergoing treatment from March to June 2005 at the Reproductive Medical Center in the Provincial Hospital of Shandong.,all of whom had signed informed consents explicitly for this study. Pick-up of immature oocytes were performed 36 hours after HCG injection in natural...