Expression Of PTEN In SH-SY5Y Cells In Which Tau Protein Was Induced Hyperphosphorylation By Okadaic Acid And Effect Of Melatonin On It

Objective: Taking tau protein hyperphosphorylation as a reflection index of development in AD (Alzheimer's Disease), the present study was designed to explore initially the expression of PTEN in experimental AD and its relationship with tau protein hyperphosphorylation in order to comprehend the pathogenesy of AD; The present paper was also designed to investigate the antagonistic effect of melatonin on AD-like cells and the relationship with tau protein hyperphosphorylation and PTEN in order to provide the theoretical foundation on clinical appliance of melatonin and its analogues. Methods:â‘ . SH-SY5Y cells were treated with OA (Okadaic acid),taking tau protein hyperphosphorylation and cell viabilities as reflection index, to establish a cell model for research on AD;â‘¡MTT assay was used to measure the viabilities in cells;â‘¢Western Blotting was used to measure the levels of phosphorylated tau protein at site of Ser396 and PTEN;â‘£Immunocytochemical staining was used to measure the intensity of phosphorylated tau protein-like immunoreactivity in SH-SY5Y cells.Results:â‘ Treatment of SH-SY5Y cells with OA caused a decrease in cell viability in a dose and time dependent manner; SH-SY5Y cells was treated with 40 nmol/L OA for various periods, the levels of phosphorylated tau...