Effects Of Anisodamine On INOS And ICMA-1 MRNA Expression In Lung And Intestine Of Rats Following Traumatic/Hemorrhagic Shock And Resuscitation

objective: To study the effects of anisodamine on iNOS and ICMA-1 mRNA Expression in Lung and Intestine of Rats following Traumatic/Hemorrhagic Shock and Resuscitation. methods: With the rat models of traumatic/hemorrhagic shock established, we randomly divided rats into three groups: normal control group(SHAM group), traumatic/hemorrhagic shock with resuscitation group (RESUS1 group)and corporation resuscitation with 654-2 -treated group(RESUS2 group) . In every group we kill rats at 24h after model established and picked the left lung-lobes and intestines tissues out , make a label in each specimen and reserved it in a low temperature (-70OC) icebox or dip in 10% formalin. Take the lung tissues out of 10% formalin, following three stages with embed,slice up and HE coloration. Two pathologist observe and calculate it for tissues damnification condition. In each group of rat according to reagent exponent, we passed the stage of abstracting total RNA,composing the cDNA,together PCR reaction and...