Neuroprotective Effects Of Cerebrolysin/NGF On Hypoxic-ischemic Injuried Cells Of Human Fetal Subventricular Zone

ObjectiveTo investigate the effects of hypoxia-ischemia(HI)on neural stem cells(NSCs), neurons, glial cells(astrocytes, oligodendrocyte progenitors and microglia cells )in human fetal SVZ and compare the different survivabilities of SVZ cells to HI,thus to explore the pathogenesis of hypoxic-ischemic brain damage (HIBD) on the cellular lever. At the same time, to investigate the protective effect of NGF/Cerebrolysin on the hypoxic-ischemic injury of SVZ cells, so provide evidence for treating HIBD with them.MethodsAcutely dissociated SVZ cells, prepared from five cases of brains human embryonic brains aborted voluntarily aged from 17 gw to 22 gw, before and after hypoxia/ischemia 10 minutes in vitro [by oxygen/glucose deprivation (OGD)[1]], were counted by staining with trypan Blue. The HI and the normal SVZ cells were cultured by four groups: NGF, Cerebrolysin, NGF/Cerebrolysin and blank control group,which was designed by two factors (A:NGF,B: Cerebrolysin) and two levers(using drug and without drug). After 4 hours, the main cells of SVZ, including NSCs, neurons, astrocytes, oligodendrocyte progenitors and microglia cells were recognized separately by their special maker nestin, MAP2, GFAP, PDGFRαand RCA120 with immunofluorescence cytochemistry. At the same time, all cell nucleuses were stained by bisbenzimide. Then compared the different percentages of SVZ cells between HI and normal groups and analyzed the effects of NGF/Cerebrolysin on HI and normal SVZ cells, HI neurons.Results...