The Effects Of Fluvastatin On LTCC(a1C),SERCA2,IP3R-1 Expression In VSMC From Spontaneously Hypertensive Rat

Objective:To investigate the effects of fluvastatin on LTCC(alc),IP3R-1, SERCA2 expression in thorax aorta VSMC from spontaneously hypertensive rats(SHR) and to explore the possible role of fluvastatin in the pathological vessel remodeling of hypertension.Methods:1. 24 12-week-old male SHR were randomized into 3 groups and treated witha placebo, benazepril(10mg·kg^-1·d^-1,ACE inhibitor), or fluvastatin (20mg. kg^-1· d^-1, 3- Hydroxy-3-methyl- glutaryl coenzyme A reductase inhibitor). Age-matched male WKY rats served as controls. Blood pressure and weight of SHR were measured once a month. Rats were killed after 18- week treatment and then wall-to-lumen area ratio of thoracic aorta were assessed by morphormetric assay. LTCC( a1c) , SERCA2, IP3R-1 mRNA in thoracic aorta were determined by RT-PCR. The levels and distribution of LTCC( alc), IP3R-1 protein were monitored by immunohistochemistry. SERCA2 protein expression was evaluated by WB analysis.2. VSMCs from 8-week old SHR were cultured to investigate (1)The effectsof two mitogens:ET-1 and PDGF on the levels of LTCC(alc), SERCA2, IP3R-1 mRNA (2) The effects of fluvastatin on the levels of LTCC( alc), SERCA2, IP3R-1 mRNA stimulated by ET-1 and PDGF.Results:Part 1: In vivo(1) The wall-to-lumen area ratio was markedly reduced by fluvastatin or benazepril treatment. There was no significant difference between fluvastatin and benazepril treated groups.(2) LTCC(alc),IP3R-1,SERCA2 mRNA manifested a marked reduction in SHRc...